Biofilm Formation in drinking water distribution
1. Formation of a surface conditioning film
It is a thin layer of organic molecules and ions
covering the adhesion surface and is formed before attachment of
microorganisms. Physical or chemical adsorption helps these processes. The
former is a reversible process and includes nonspecific bonds such as van der
Waals with low adsorption energies. Whereas latter is a nonreversible and
includes specific chemical bonds such as electrostatic, covalent and hydrogen
bonds, dipole interactions, and hydrophobic interactions with higher adsorption
energies. In this process several adsorbed molecular layers are formed which
determines the strength of the biofilm adhesion. Besides, the conditioning film
helps bacteria to attach on the surface of pipeline by neutralizing flow
velocity and do also provide nutrient for bacteria (Marshall, 1996).
2. Initial adhesion of “pioneer” microorganisms
to the surface;
Planktonic (free floating) microorganisms are
transported towards the surface either by fluid dynamics, gravitational forces
and Brownian motion, or by migration through active cell motility (e.g. flagella).
The surface electrostatic charge and hydrophobic interactions are also found to
affect this approaching and adhesion (Mueller, 1996).
The first arriving free floating bacteria adhere to
the surface initially through weak, reversible electrostatic attraction and van
der Waals forces. If not immediately separated from the surface, the bacteria
are anchored more permanently by developing stronger bonds for the attachment
to the surface for example, by active cell biosynthesis of EPS and by chemical
forces. These cells become irreversibly adsorbed. Different mechanisms had been
proposed to explain the bacteria attachment to surfaces with different
hydrophobicity character (Marshall,
1996).
3. Biofilm growth
The attached bacteria start to grow, excrete organic
polymers, and initiate the formation of the biofilm matrix. Tolker-Nielsen et
al., (2000) observed that the first micro-colonies formed were
mono-species. Further attachment of planktonic bacteria and of inorganic
particles will contribute to a structurally heterogeneous biofilm growth, as
well as, migration of attached bacteria between and inside of micro-colonies.
During this phase, bacteria detachment events occur although at a lower extent
compared to the growth rate.
4. Biofilm maturation - equilibrium between
accumulation and detachment.
Mature biofilm are composed of an organized consortia
of microorganisms embedded in an organic matrix that protects the bacteria. The
structure of a mature biofilm depends on the microbial composition, EPS
production, the nutrient availability, hydrodynamic conditions and temperature.
In a biofilm several processes may occur simultaneously: bacterial detachment
into water, attachment of planktonic bacteria, growth, death etc. In a mature
biofilm these processes are at equilibrium and the attached cells per unit
surface area are constant with time although with periodic fluctuations. At
this phase, the biofilm reach the highest thickness that does depends on the
hydraulic conditions, the mass transport and the biofilm cohesion (Momba et
al., 2002).
According to Momba et al., (2002) several
factors were observed to promote detachment of biofilm portions or of isolated
bacteria. These factors are the following:
Sloughing off:
The increase of shear stress, alternating flow conditions and abrasion due to
particle collisions promote sloughing off of biofilm pieces that were not well
cohesive.
Starvations of
bacteria: It does promote size
reduction, and increase of bacteria fragmentation and of motility which will
increase biofilm detachment.
The increase
of nutrients: it promoted the release of bacterial cells up to 80 % the
total attached ones. This biofilm dispersion phenomenon was associated with
increased expression of flagella genes.
Chelating
agents: A chemical change in the EPS due to presence of chelating agents
(Ca2+) that will reduce the cohesive strength of the attached cells.
Surfactants:
The excretion of surface-modified products (surfactants) by certain bacteria
may promote detachment.
Signaling
molecules: In biofilms the excretion of certain signaling molecules induced
the detachment events.
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